Journal: bioRxiv
Article Title: Extracellular Vesicles from Inflammation-Primed Adipose-Derived Stem Cells Enhance Achilles Tendon Repair by Reducing Inflammation and Promoting Intrinsic Healing
doi: 10.1101/2023.01.31.526532
Figure Lengend Snippet: Characterization of extracellular vesicles from inflammation-primed adipose-derived stem cells (iEVs). (A) A representative transmission electron microscopy image of iEVs (indicated with white arrows); scale bar = 100 nm. (B) Representative nanopartical tracking analysis of iEVs. (C) Representative flow cytometry analysis of iEVs sorted with microbeads coupled with (green) or without (grey) biotinylated antibodies against indicated exosome markers.
Article Snippet: CD9+/CD63+/CD81+ iEVs were separated from bulk iEVs with Exo-Flow streptavidin magnetic microbeads (System Biosciences) coupled with biotinylated human anti-mouse CD63 (Miltenyi 130-125-989), rat anti-mouse CD9 (Miltenyi 130-101-961), and hamster anti-mouse CD81 (Miltenyi 130-101-966) antibodies according to manufacturer’s instructions.
Techniques: Derivative Assay, Transmission Assay, Electron Microscopy, Flow Cytometry